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KMID : 0377619770330010033
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Korean Jungang Medical Journal
1977 Volume.33 No. 1 p.33 ~ p.39
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The in vivo Distribution of ^(131)I-RISA in Toxocara canis, Ancylostoma caninum and Clonorchis sinensis
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金相俊/Kim, Sang-June
鄭坪林/李根泰/高昌俊/Coe, Chang-Jun/Chung, Pyung-Rim/Lee, Keun-Tae
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Abstract
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Most of the parasites in nemathelminthes and platyhelrninthes have highly developed reproductive organs as ´compared with their digestive organs, and deposit heavily protein-rich eggs. One female adult of Ascaris lumbricoides lays more than 200, 000 eggs per a day, a female of hookworm more than 20, 000 eggs and more than 4,000 eggs from an adult of leaf-like liver fluke with length of 1. 0 to I.5cm. It is considered that these eggs-depositing parasites may take nutrients not only through degenerated mouth part but also through any other absorption mechanism, especially for the compensation of protein loss due to egg deposition
From this viewpoint, an autoradiographic study was carried out for investigating the intake mechanism of protein into the above-mentioned parasites.
Toxocara canis and Ancylostonza caninum were infected to the dogs, and Clonorchis sinensis was infected to the rabbits for the present study. A serum albumin for radioisotope, 1311-RISA, was also injected intravenously into each host, which was sacrificed 48 hours after injection The parasites were removed from the sacrificed hosts, and then the quantitative distribution of radioactivity were counted by Well Type Scintillation Counter from the parasites.
The results are as follows;
1. Toxocara canis
The dog ascaris harboring freely in the intestinal lumen of the host takes protein from the host through the cuticle as well as the primitive mouth :_. Part. Absorption amount of, serum protein was 1.2m; Ci per gm. Of body weight of the parasite and the absorption rate .was. 0.6%. The results indicate that the parasites take protein mainly from the contents of intestinal´ lumen regardless of direct absorption from the intestinal wall of the host.
2. Ancylostoma caninum
Canine hookworm intakes serum protein from the host only with its mouth part. Absorption amount .bf serum protein was 100.8 mpCi per gm. Of body weight of a parasite and 50.4% of absorption rate. Absorption amount of serum protein was actually 85 times higher than that of Toxocara canis. It is showed that blood-sucking through the mouth part with 3 pairs of teeth, is enough for protein metabolism in the canine hookworm.
3. Clonorchis sinensis
The liver fluke inhabiting in the bile duct of the host liver, intook serum protein of the host with the oral sucker and integument. Absorption amount of serum protein was 60.0 mpCi per gm. Of body weight of the worm. The absorption rate (30.0%) was 50 times higher than that of Toxocara canis.
It is suggested that serum protein is intaken from bile juice and wall of the host bile duct through the integument as well as the oral sucker of the liver fluke.
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